Stabilitas Fisikokimia dan Sifat Antipenuaan Kulit Formula Krim Berbahan Aktif Alami (Physicochemical stabilities and Skin Antiaging Properties of Bioaktive-based Cream Formulas)

Abstract

The purpose of this study was  to analyze the physicochemical stabilities  and in vivo antiaging  properties of the cream formulas of F1, F2, and F3 which contained the active compound of 0.1% 0.18, and 0.36%, respectively. The active compound was the combination of  ethyl acetate fraction of  Toona sinensis, Centella asiatica, and Acacia mangium  leaf (1:2:1). For physicochemical stability tests (color, odor, acidity, and antioxidant activity), the creams were stored for 21 days in the refrigerator, room temperature,   and extreme temperature (±40 ⁰C). For in vivo antiaging test,  the mice skin  were smeared with cream and UV irradiated for 15 minutes per day. After two and four weeks, the mice skins were  biopsied. The results showed that the physicochemical properties of creams stored in the refrigerator were  stable. The storage of creams at room and  extreme temperature decreased the physicochemical properties.  The application of cream formulas on mice skin after two to four weeks were able to increase the thickness of epidermis, but has not been able to increase the collagen skin of mice.

Keywords: Acacia mangium, Centella asiatica, ethyl acetate fraction of leaf, physicochemical and antiaging properties of cream, Toona sinensis