Characterization of Cuticle Layer of Ilex latifolia

Jun-ichi Azuma, Shuntaro Tsubaki, Rike Yudianti, Myrtha Karina

Abstract


Cuticular membranes were isolated from the upper and lower surfaces of Ilex latifolia leaves and their morphological, chemical compositional and mechanical properties were characterized.  The upper cuticular membrane contained 18.6% wax, 53.5% cutin and 17.5% cutan with low contaminating carbohydrates (10.4%), while the lower cuticular membrane had the values of 17.9%, 49.2%, 15.8% and 17.1%.  Both membranes were rich in aliphatic CH2 groups, and the main monomer of the cutin in the upper cuticular membrane was 9(10),18-dihydroxyhexadecanoic acid while 9,10,18-trihydroxyoctadecanoic acid in the lower membrane. Mechanical analyses indicate clear orientation differences in breaking stress and strain between the two membranes with higher breaking stress in the upper cuticular membrane and in the longitudinal direction parallel to the midvein. Additive such as a cellulosic hydrogel from Salvia viridis to make a composite influenced the mechnical properties of the cuticular membranes. Mechanical properties of the isolated cuticular membranes may be more influenced by their morphological properties than chemical compositions; thick and smooth structure of the upper cuticular membrane, while wrinkled and scattered stoma-rich structure of the lower cuticular membrane.


Keywords


cuticular membrane; cutin; chemical properties; mechanical properties; Ilex latifolia.

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References


Azuma, J.; M. Ohashi; M. Sakamoto. 2006. Salty Wind Torelance of Mangroves, Seminar Nasional Mapeki IX, Banjarbaru, Indonesia, August 11-13.

Baker, E.A.; P.J. Holloway. 1970. The Constituent Acids of Angiosperm Cutins, Phytochemistry 9: 1557-1562.

Fournier, E. 2005. Mono- and Oligosaccharides: In Handbook of Food Analytical Chemistry, Water, Proteins, Enzymes, Lipids, and Carbohydrates. Wrolstad, R.E. et al. Eds. A John Wiley & Sons, Inc., Publication: 653-660.

Holloway, P.J. 1982. The Chemical Constitution of Plant Cutins: In The Plant Cuticle. Cutler, D.F.; K.L. Alvin; C.E. Price, Eds. Academic Press, New York: 45-85.

Jeffree, C.E. 1996. Structure and Ontogeny of Plant Cuticles: In Plant Cuticles Anintegrated Functional Approach. Kerstiens, G., Ed. BIOS Scientific Publishers, Oxford: 33-82.

Luque, P.; A. Heredia. 1994. Glassy State in Plant Cuticles during Growth. Z. Naturforsch. 49c: 273-275

Melton, L.D.; B.G. Smith. 2005. Determination of the Uronic Acid Content of Plant Cell Walls using a Colorimetric Assay: In Handbook of Food Analytical Chemistry, Water, Proteins, Enzymes, Lipids, and Carbohydrates. Wrolstad, R.E. et al. Eds. A John Wiley & Sons, Inc., Publication: 735-738.

Nawrath, C. 2002. The Biopolymers Cutin and Suberin: In The Arabidopsis Book. Somerville, C.R.; E.M. Meyerowitz, Eds. ASPB Press, Rockville, MD, USA (http://www.aspb.org/publications/Arabidopsis/).

Pacchiano, R.A.; W. Jr. Sohn; V.L. Chlanda; J.R. Garbow; R.E. Stark. 1993. Isolation and Spectral Characterization of Plant-cutcle Polyesters. J. Agric. Food Chem. 41: 78-83.

Shiroya, M.; S. Hattori. 1955. Studies on the Browning and Blackening of Plant Tissues, III. Occurrence in the Leaves of Dahlia and Several Other Plants of Chlorogenic Acid as the Principal Browning Agent, Phsiologia Plantarum 8: 358-369.

Villena, J.F.; E. Domínguez; A. Heredia. 2000. Monitoring Biopolymers Present in Plant Cuticles by FT-IR Spectroscopy. J. Plant Physiol. 156: 419-422.

Walton, T.J.; P.E. Kolattukudy. 1972. Determination of the Structures of Cutin Monomers by A Novel Depolymerization Procedure and Combined Gas-Chromatography and Mass Spectrometry, Biochemistry 11: 1885-1897.

Yudianti, R.; L. Indrarti; J. Azuma; M. Sakamoto. 2005. Cellulose-hemicellulose Present in Hydrocolloids from Salvia spp., Towards Ecology and Ecomomy Harmonization of Tropical Forest Resources, Proceedings of the 6th International Wood Science Symposium, Bali, Indonesia, p. 273-277.

Zlotnik-Mazori, T.; R.E. Stark. 1988. Nuclear Magnetic Resonance Studies of Cutin, An Insoluble Plant Polyester. Macromolecules 21: 2412-2417.




DOI: https://doi.org/10.51850/wrj.2010.1.1.56-63

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